Reagents and instruments for immunology, cell biology and molecular biology.

α-Ketoglutarate (α-KG) Fluorometric Assay Kit

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Cat# E-BC-F047-96T

Size : 96T

Brand : Elabscience

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Detection Principle	α-Ketoglutaric acid and alanine can be combined with fluorescent probe products under the action of a series of enzymes. The content of α-ketoglutaric acid in samples can be determined by measuring the fluorescence value.
Synonyms	α-KG
Sample Type	Serum,Plasma,Urine,Animal tissue,Cell sample
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area	Tricarboxylic Acid（TCA）Cycle , Cuproptosis , Energy Metabolism
Other Reagents Required	Normal saline (0.9% NaCl)
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.60 μmol/L
Detection Range	0.60-100 μmol/L
Precision	inter-assay CV: 0.04 \| intra-assay CV: 0.02
Assay Time	35 min

Cat.No.	Product Name	Sizes
E-BC-K083-M	α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit	96T
E-BC-K178-M	Citrate Synthase (CS) Activity Assay Kit	96T , 48T
E-BC-K650-M	Pyruvate Dehydrogenase (PDH) Activity Assay Kit	96T
E-BC-K651-M	NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit	96T
E-BC-K652-M	Acetyl-CoA Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat kidney tissue homogenate	1
10% Rat lung tissue homogenate	1
10% Porcine heart tissue homogenate	1
10% Rat brain tissue homogenate	1
10% Mouse liver tissue homogenate	1
10^6 Jurkat cell	1
Human serum	1
Rat serum	2-3

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.