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> Tyramide Amplification Kit with HRP Streptavidin and CF®488A Tyramide

Tyramide Amplification Kit with HRP Streptavidin and CF®488A Tyramide

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Cat# 33002

Size : 1kit

Brand : Biotium

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Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide
Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide
Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide
Tyramide Amplification Kit with HRP Streptavidin and CF® Dye or Biotin Tyramide
Storage Conditions

Store at -10 to -35 °C, Protect dye component from light
Protocols

PI-Tyramide Kits

SDS

SDS Tyramide Amplification Kit

Supporting Documents

Tyramide Signal Amplification Flyer

CF-Dye-Brochure

Product Description

This kit contains reagents for performing tyramide signal amplification (TSA) using streptavidin HRP conjugate and fluorescent CF® Dyes or biotin to increase immunofluorescence signal.

Features

  • Increase sensitivity by up to 100-fold
  • Use lower primary antibody concentrations
  • Boost signal in challenging samples, like human FFPE tissues
  • Choose from 6 bright & photostable CF® dyes or biotin

Kit Components:

  • CF® dye or biotin-tyramide stock solution
  • HRP Streptavidin
  • BSA for blocking
  • Tyramide Amplification Buffer Plus

    *

  • 30% Hydrogen Peroxide

    *

Note: Endogenous biotin blocking reagents (not provided) may be required for streptavidin/biotin detection in cells or tissues.

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ.  The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to conventional avidin-biotin detection. Multiple rounds of tyramide signal amplification can be performed for multiplex detection; to learn more, see our

Tech Tip: Multicolor Fluorescence Imaging using Tyramide Amplification Kits

.

Learn more about

tyramide signal amplification.

These kits include your choice of 6 CF® dye tyramides or biotin tyramide. CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Learn more about

CF® dyes

.

Also see our

Tyramide Amplification Kits with HRP Goat Anti-Mouse

and

Tyramide Amplification Kits with HRP Goat Anti-Rabbit

.

Each kit contains reagents for staining 50-150 slides; the actual number of samples that can be stained will depend on staining volumes used. 

Tyramide Amplification Buffer

,

CF® Dye Tyramides

, and other tyramide compounds are also available separately. See our

full selection of tyramide products

.

Tyramide Signal Amplification Kits

Tyramide Label Ex/Em Secondary conjugate Catalog no.
CF®488A 490/515 nm

Goat anti-mouse HRP

33000

Goat anti-rabbit HRP

33001

Streptavidin HRP

33002
CF®543 541/560 nm

Goat anti-mouse HRP

33003

Goat anti-rabbit HRP

33004

Streptavidin HRP

33005
CF®568 562/583 nm

Goat anti-mouse HRP

33006

Goat anti-rabbit HRP

33007

Streptavidin HRP

33008
CF®594 593/614 nm

Goat anti-mouse HRP

33009

Goat anti-rabbit HRP

33010

Streptavidin HRP

33011
CF®640R 642/662 nm

Goat anti-mouse HRP

33012

Goat anti-rabbit HRP

33013

Streptavidin HRP

33014
CF®680R 680/701 nm

Goat anti-mouse HRP

33015

Goat anti-rabbit HRP

33016

Streptavidin HRP

33017
Biotin-XX N/A

Goat anti-mouse HRP

33018

Goat anti-rabbit HRP

33019

Streptavidin HRP

33020

*Note: Older versions of this kit contain Ready-to-Use Tyramide Amplification Buffer (component 22027-25mL) without a separate vial of hydrogen peroxide. Download the product information sheet for detailed protocols. The SDS for the older version of the kit can be downloaded here:

SDS-Tyramide-Amplification-Kit with 22027

.

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