Triiodothyronine indes, Free (FT3) BioAssay™ ELISA Kit (Goat)

Sample Type:|Serum and plasma||Intended Use:|For the quantitative determination of goat free tri-iodothyronine indes(FT3) concentrations in serum and plasma.||Sensitivity:|0.38pmol/l||Range:|2-32pmol/L||Specificity|This assay has high sensitivity and excellent specificity for detection of goat FT3. No significant cross-reactivity or interference between goat FT3 and analogues was observed.||Test Principle:|This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of Free-T3 in the sample. The color development is stopped and the intensity of the color is measured.||Kit Components:|Assay plate, 1x96 wells|Standard, 5x1ml|Conjugate, 1x6ml|HRP-avidin, 1x6ml|Wash Buffer (20X), 1x15ml|Substrate A, 1x7ml|Substrate B, 1x7ml|Stop Solution, 1x7ml|Adhesive Strip (For 96 wells), 4x strips|Instruction manual||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.