TransDetect® Double-Luciferase Reporter Assay Kit

Cat# FR201-01

Size : 50rxns

Brand : TransGen Biotech

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TransDetect® Double-Luciferase Reporter Assay Kit

Catalog Number: FR201-01-V2

Price:Please inquire first

Specification:
50 rxns
200 rxns

Product Details

Firefly luciferase and Renilla luciferase can catalyze the oxidation of luciferin or coelenterazine to form oxyluciferin or  coelenteramide, respectively, and produce bioluminescence in the process. TransDetect®Double-Luciferase Reporter Assay Kit first uses luciferin as a substrate to detect the activity of the firefly luciferase  reporter gene. Then, while quenching the fluorescence reaction, the activity of the Renilla luciferase reporter gene was detected by  using coelenterazine as a substrate. It has the characteristics of rapid detection, high sensitivity, wide detection range, and no  interference with the endogenous activity of cells.

Storage

The kit stored at -20°C for one year. Cell Lysis Buffer can be stored at -20°C for one year. Prepared Luciferase Reaction  Reagent should be stored in aliquots in dark at -20°C for one month or at -70°C for one year.

Shipping

Dry ice (-70℃)

Product Composition

Component

FR201-01-V2 (50 rxns)FR201-02-V2 (200 rxns)
Luciferase Reaction Buffer 5 ml 20 ml 
Luciferase Reaction Substrate (Lyophilized)   1 vial 4 vials
Luciferase Reaction Buffer II5 ml 20 ml 
Luciferase Reaction Substrate II (50×)100 μl 400 μl 
Cell Lysis Buffer (5×)5 ml20 ml


References

1 Ji X, Du Y, Li F, et al. The basic helix‐loop‐helix transcription factor, Os PIL 15, regulates grain size via directly targeting a purine permease gene Os PUP 7 in rice[J]. Plant Biotechnology Journal, 2019.(IF 6.30)

2 Li Y, Feng D, Wang Z, et al. Ischemia-induced ACSL4 activation contributes to ferroptosis-mediated tissue injury in intestinal ischemia/reperfusion[J]. Cell Death & Differentiation, 2019.(IF 8.00)

3 Liu F, Xu Y, Chang K, et al. The long noncoding RNA T5120 regulates nitrate response and assimilation in Arabidopsis[J]. New Phytologist, 2019.(IF 7.43)

4 Qi Y, Ding L, Zhang S, et al. A plant immune protein enables broad antitumor response by rescuing microRNA deficiency[J]. Cell, 2022.(IF 41.58)

5 Cheng Q, Wang J, Li M, et al. CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease[J]. Redox Biology, 2022.(IF 10.78)

6 Di Y, Jing X, Hu K, et al. The c-MYC-WDR43 signalling axis promotes chemoresistance and tumour growth in colorectal cancer by inhibiting p53 activity[J]. Drug Resistance Updates, 2023.(IF 22.84)

7 Jia X, Wang L, Zhao H, et al. The origin and evolution of salicylic acid signaling and biosynthesis in plants[J]. Molecular Plant, 2023.(IF 21.94)

8 Liu Y, Zhang S, Chen L, et al. The molecular mechanism of miR-96-5p in the pathogenesis and treatment of polycystic ovary syndrome[J]. Translational Research, 2023.(IF 10.17)

9 Zhou J, Ge Q, Wang D, et al. Engineering a modular double-transmembrane synthetic receptor system for customizing cellular programs[J]. Cell Reports, 2023.(IF 9.99)

10 Cui Y, Lyu X, Ding L, et al. Global miRNA dosage control of embryonic germ layer specification[J]. Nature, 2021.(IF 42.00)


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