SEAP Reporter Gene Assay Kit
Cat# CA040
Size : 288assays
Brand : Canvax Biotech
SEAP Reporter Gene Assay Kit
For a really Easy, Sensitive & Fast Assay, Luminescence based, Measurement the levels of SEAP in the Culture medium
Secreted Alkaline Phosphatase (SEAP) Reporter Gene is a really easy, sensitive and fast assay that utilizes enzyme activity of alkaline phosphatase to dephosphorylate the chemiluminescent alkaline phosphatase substrate CSPD into an unstable dioxetane anion which decomposes and emits light.
SEAP encodes a truncated form of the placental enzyme that lacks the membrane anchoring domain, thereby allowing the protein to secrete efficiently from transfected cells.
Changes in levels of SEAP activity detected in the culture medium are directly proportional to changes in intracellular concentrations of SEAP mRNA and protein.
Detailed information:
- Convenient: a single set of cells is used for both the SEAP assay and another purpose.
- Time-saving protocol: results in 55 minutes due the elimination of cell lysate preparation.
- Cost-effective: allows performance directly in a microtiter plate.
- Sensitive: assayed even in low cell concentrations.
- Secreted from transfected cells into the culture medium.
– 3 x 96 W Solid Plate (white)
– 3 units of lid
– 50 μL Alkaline Phosphatase Standard
– 15 mL SEAP Substrate (Luminescence)
Datasheet MSDS- Measurement the levels of SEAP in the culture medium of transfected cells.
- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Precautions: Some components of this kit are potentially carcinogenic or corrosive; it is advisable to work in a hood and to wear glasses, gloves and a mask.
- Shipping: Ambient Temperature.
- Storage: 4 ºC, except Component solution containing CSPD® Substrate (2-6 °C), Placental Alkaline Phosphatase Positive Control (-20 °C).
- Recommendations: Protect SEAP Substrate from light.
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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