Recombinant SARS-CoV-2 NSP12 protein

Cat# PKSV030327-100

Size : 100ug

Brand : Elabscience

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Synonyms RNA-directed RNA polymerase, RdRp, SARS-CoV 2 nsp12
Species SARS-CoV-2
Expression Host E.coli
Sequence Asp4891-Val5212
Accession QHD43415.1
Calculated Molecular Weight 64.0 kDa
Observed Molecular Weight 60 kDa
Tag N-Gst
Bio-activity Not validated for activity
Purity > 90 % as determined by reducing SDS-PAGE.
Endotoxin Please contact us for more information.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5
Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.
Background The RdRp of SARS-CoV-2 is composed of a catalytic subunit known as nsp12 as well as two accessory subunits, nsp8 and nsp7. The structure of this RdRp has recently been reported; it is highly similar to the RdRp of SARS-CoV, a zoonotic coronavirus that spread into the human population in 2002. The nsp12 subunit contains an N-terminal nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain, an interface domain and a C-terminal RdRp domain. The RdRp domain resembles a right hand, comprising the fingers, palm and thumb subdomains that are found in all single-subunit polymerases. Subunits nsp7 and nsp8 bind to the thumb, and an additional copy of nsp8 binds to the fingers domain. Structural information is also available for nsp8–nsp7 complexes.
Other Clones

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Biotinylated

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