Size : 5 ug
| Symansis' purified Lymphotoxin-alpha/TNF-betahcx consists of up to 30% carbohydrate. Symansis' Lymphotoxin-alpha/TNF-betahcx is N-glycosylated and O-glycosylated, shown by a drop in observed MW on SDS-PAGE after treatment with PNGase F, and a further drop in observed MW on SDS-PAGE after treatment with a glycosidase cocktail. Densitometry of protein isoforms visualized by 2-DE. Spot trains are normal in human proteins and highlight the presence of multiple isoforms which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein. 1D SDS-PAGE of Symansis' hcx protein before and after treatment with glycosidases to remove oligosaccharides. A drop in the observed MW after treatment indicates the presence of glycosylation. 1. McDevitt H, et al. (2002) Arthritis Res. 4: S141-52. |
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