Recombinant Human IL-11
Cat# P1125-500ug
Size : 500ug
Brand : APExBIO Technology
Recombinant Human IL-11
Interleukin-11 (IL-11) is encoded by the IL11 gene and is a member of IL-6 superfamily, distinguished based on their use of the common co-receptor gp130. It is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. IL-11 receptor activation requires formation of a complex of two IL-11 molecules with two molecules of the ligand-binding IL-11Rα subunit and two molecules of the expressed cell signaling β subunit, gp130. The functions of IL-11 are that it directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells, and induces megakaryocyte maturation resulting in increased platelet production.
Reference:
1. McKinley D, Wu Q, Yang-Feng T, et al. 1992. Genomics. 13:814-9.
2. Paul SR, Bennett F, Calvetti JA, et al. 1990. Proc Natl Acad Sci U S A. 87:7512-6.
3. Barton VA, Hall MA, Hudson KR, et al. 2000. J Biol Chem. 275:36197-203.
| Gene ID | 3589 |
| Accession # | P20809 |
| Alternate Names | Interleukin-11, Adipogenesis inhibitory factor, AGIF, Oprelvekin, IL11. |
| Source | Escherichia coli. |
| M.Wt | Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids. |
| AA Sequence | MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. - 1 month, 2 to 8°C under sterile conditions after reconstitution. - 3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine B9-11 cells is less than 1 ng/ml, corresponding to a specific activity of >1.0×106 IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & MSDS
- View current batch:
- Purity >95% by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/µg of rHuIL-11 as determined by LAL method.