Reactive Oxygen Species (ROS) Fluorometric Assay Kit
Cat# E-BC-K138-F-96T
Size : 96T
Brand : Elabscience
| Detection Principle | DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species. |
| Synonyms | DCF;DCFH;DCFH-DA;ROS |
| Sample Type | Fresh tissue,Cultured cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader, Fluorescence microscope, Flow cytometry (Ex/Em=500 nm/525 nm) |
| Research Area | Oxidative Stress , Ferroptosis , Metabolic Diseases , Energy Metabolism |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Assay Time | 1 h |
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