Reagents and instruments for immunology, cell biology and molecular biology.

Phosphate Colorimetric Assay Kit(Malachite Green Method)

Print

Cat# E-BC-K832-M-96T

Size : 96T

Brand : Elabscience

Request more information

Detection Principle	Phosphorus is an important mineral, one of the essential nutrients for all animals, and is essential for maintaining phosphate homeostasis. Malachite green method is a sensitive method for phosphate analysis. The principle is that the compounds formed by phosphoric acid and molybdate under acidic conditions will form color substances with malachite green. The depth of color is proportional to the content of phosphate. The phosphorus content can be calculated indirectly be measuring the OD value at 636 nm.
Sample Type	Serum,Plasma,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (630-650 nm)
Research Area	Inorganic Ions , Oxidative Stress
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.001 mmol/L
Detection Range	0.001-0.20 mmol/L
Precision	inter-assay CV: 0.08 \| intra-assay CV: 0.04
Assay Time	35 min

Cat.No.	Product Name	Sizes
E-BC-K831-M	ATPase Activity Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	15-25
10% Rat heart tissue homogenate	15-25
10% Rat spleen tissue homogenate	15-25
10% Rat brain tissue homogenate	15-25
10% Rat kidney tissue homogenate	15-25
10% Mouse heart tissue homogenate	15-25
10% Mouse lung tissue homogenate	15-25
10% Mouse ovarian tissue homogenate	50-70
Rat serum	8-12
Rat plasma	8-12
Human plasma	8-12

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.