Reagents and instruments for immunology, cell biology and molecular biology.

p53 (Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Cellular Tumor Antigen p53, Phosphoprotein p53, Antigen NY-CO-13, LFS1) (HRP)

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Cat# P1001-26R-HRP-100ul

Size : 100ul

Brand : US Biological

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P1001-26R-HRP p53 (Tumor Protein p53, Tumor Suppressor p53, TP53, TRP53, Cellular Tumor Antigen p53, Phosphoprotein p53, Antigen NY-CO-13, LFS1) (HRP)

Clone Type

Polyclonal

Host

mouse

Source

human

Swiss Prot

P04637

Isotype

IgG2a

Grade

Affinity Purified

Applications

E IHC IP WB

Crossreactivity

Bo Eq Fe Hu

Shipping Temp

Blue Ice

Storage Temp

-20°C

p53 is a 53kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.||Applications:|Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot. Other applications have not been tested.||Recommended Dilution:|Immunohistochemistry: Frozen.|Immunohistochemistry (Paraffin): 1:1000|Western Blot: 1:1000|Note: Requires antigen retrieval; sodium citrate buffer pH 6.0 is recommended . Recommended positive control tissue: Colon or breast carcanoma.|Optimal dilutions to be determined by the researcher.||Recommended Secondary Reagents: |I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo|I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo||Recommended Negative Controls (IHC): |I1904-83P1: IgG2a Negative Control||Hybridoma: |P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG2a|Clone No: DO-1|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography.|Immunogen: Recombinant protein corresponding to human p53.|Specificity: Recognizes the human p53 tumor suppressor protein, binding to both wild type and mutant forms. Rcognizes an epitope at the N-terminal end of p53 between amino acids 20-25. |Species Crossreactivity: Bovine, equine and feline (2). Does not react with mouse or rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Recombinant protein corresponding to human p53.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).

Purity

Purified by Protein G affinity chromatography.

Specificity

Recognizes the human p53 tumor suppressor protein, binding to both wild type and mutant forms. Rcognizes an epitope at the N-terminal end of p53 between amino acids 20-25. |Species Crossreactivity: Bovine, equine and feline (2). Does not react with mouse or rat.

References

1. Vojtesek, B. et al. (1992). An immunohistochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53. J. Immunol. Meth. 151: 237-244. . 2. Sironi, G. et al . (1999). p53 protein expression in conjunctival squamous cell carcinomas of domestic animals. Veteriniary Ophthalmology. 2: 227 - 231. 3. Levesque, M. et al . (1995). Time-resolved immunofluorometric assay of p53 protein. Clin. Chem. 41(12): 1720 - 1729.