Nitric Oxide Fluorometric Assay Kit

Nitric oxide is essential in neurotransmission, vascular regulation, immune response and apoptosis. Because Nitric oxide is rapidly converted into nitrite and nitrate, the NO production can be quantitatively determined by the total concentration of nitrite and nitrate. This kit is a simple and accurate two-step measurement of total nitrate/nitrite concentration. Nitrate reductase converts nitrate into nitrite first, and then nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The total nitric oxide production is proportional to the fluorescent intensity. The kit has been tested with culture media, plasma, and tissue homogenates.