Reagents and instruments for immunology, cell biology and molecular biology.

NADP+/NADPH Colorimetric Assay Kit (WST-8)

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Cat# E-BC-K803-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle	Detect total content of NADP⁺ and NADPH: Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP⁺ is reduced to NADPH during this reaction. NADPH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NADP⁺ and NADPH can be quantified by measure the OD value at 450 nm. Detect NADPH: After treating sample, heat at 60℃ water bath for 30 min. the NADP⁺ of the sample is decomposed and only NADPH remains. NADPH reduces WST-8 to form Formazan, and the amount of NADPH is determined by measure the OD value at 450 nm. Detect NADP⁺ and NADP+/NADPH: The content of NADP⁺ and the ratio of NADP⁺/NADPH in the sample can be obtained according to the total content of NADP⁺ and NADPH obtained of the first two steps as well as the separate content of NADPH.
Synonyms	NADP;NADPH
Sample Type	Animal tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (450 nm)
Research Area	Oxidative Stress , Metabolic Diseases , Glycolysis And Lipid Metabolism , Cuproptosis , Disulfidptosis
Other Reagents Required	Ultrapure water
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.02 μmol/L
Detection Range	0.02-5.0 μmol/L
Precision	inter-assay CV: 5.5% \| intra-assay CV: 2.1%
Assay Time	1 h 10 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Jurkat cells	1
Mark cells	1
HCT116 cells	1
293T cells	1
10% Mouse kidney tissue homogenate	1
Hela cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.