Reagents and instruments for immunology, cell biology and molecular biology.

NADH (disodium salt) [606-68-8]

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Cat# HY-F0001-500mg

Size : 500mg

Brand : MedChemExpress

NADH disodium salt (Disodium NADH) is an orally active reduced coenzyme. NADH disodium salt is a donor of ADP-ribose units in ADP-ribosylaton reactions and a precursor of cyclic ADP-ribose. NADH disodium salt plays a role as a regenerative electron donor in cellular energy metabolism, including glycolysis, β-oxidation and the tricarboxylic acid (TCA) cycle.

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NADH disodium salt Chemical Structure

CAS No. : 606-68-8

Based on 4 publication(s) in Google Scholar

Other Forms of NADH disodium salt:

NADH Get quote
NADH disodium hydrate Get quote
β-Nicotinamide adenine dinucleotide reduced dipotassium Get quote

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

NADH is unstable under acidic conditions but it is stable under alkaline conditions^[2].
NADH (0-1 mM; 0-12 h) increases NAD⁺ levels in various mammalian cell lines^[3].
NADH (1 mM; 24 h) causes low toxicity and protects cells from genotoxicity^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

NADH (5 μmol/mouse; i.p.; once) increases urinary excretion of nicotinamide and its metabolites in mice^[2].
NADH (500 mg/kg; i.g.; once) promotes alcohol metabolism and prevents or ameliorates early liver injury caused by acute alcohol exposure in ethanol-loaded mice^[3].
NADH (1000 mg/kg; i.p.; once) enhances tissue NAD⁺ levels in male C57BL/6J mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ICR mice^[2]
Dosage:	5 μmol/mouse
Administration:	Intraperitoneal injection or oral administration, once
Result:	Produced significant increases in urinary excretions of nicotinamide (Nam) with intraperitoneal injection. Oral administration did not produce any increases in Nam or its metabolites.

Animal Model:	Male C57BL/6J mice^[3]
Dosage:	500 mg/kg
Administration:	Intragastric administration, 15 min before ethanol administration
Result:	Significantly increased blood acetaldehyde levels in mice administered with alcohol between 30 min and two hours. Significantly reduced the acetaldehyde in the blood after two hours. Inhibited the decrease of NAD⁺/NADH redox ratio in hepatocytes.

Molecular Weight

709.40

Formula

C₂₁H₂₇N₇Na₂O₁₄P₂

CAS No.

606-68-8

Appearance

Solid

Color

Off-white to yellow

SMILES

O[C@H]1[C@@H](O)[C@H](N2C=C(C(N)=O)CC=C2)O[C@@H]1COP(OP(OC[C@@H]3[C@@H](O)[C@@H](O)[C@H](N(C4=NC=N5)C=NC4=C5N)O3)(O[Na])=O)(O[Na])=O

Structure Classification

Others

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (140.96 mM)

DMSO : 100 mg/mL (140.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.4096 mL	7.0482 mL	14.0964 mL
5 mM	0.2819 mL	1.4096 mL	2.8193 mL
10 mM	0.1410 mL	0.7048 mL	1.4096 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.52 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.52 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (140.96 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (286 KB) SDS (393 KB)

COA (261 KB) HNMR (315 KB) RP-HPLC (235 KB)

Handling Instructions (2659 KB)

References

[1]. Ying W. NAD+ and NADH in cellular functions and cell death. Front Biosci. 2006 Sep 1;11:3129-48. [Content Brief]

[2]. Kimura N, et al. Comparison of metabolic fates of nicotinamide, NAD+ and NADH administered orally and intraperitoneally; characterization of oral NADH. J Nutr Sci Vitaminol (Tokyo). 2006 Apr;52(2):142-8. [Content Brief]

[3]. Wu K, et al. NADH and NRH as potential dietary supplements or pharmacological agents for early liver injury caused by acute alcohol exposure. Journal of Functional Foods, 2021, 87: 104852.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.4096 mL	7.0482 mL	14.0964 mL	35.2410 mL
	5 mM	0.2819 mL	1.4096 mL	2.8193 mL	7.0482 mL
	10 mM	0.1410 mL	0.7048 mL	1.4096 mL	3.5241 mL
	15 mM	0.0940 mL	0.4699 mL	0.9398 mL	2.3494 mL
	20 mM	0.0705 mL	0.3524 mL	0.7048 mL	1.7621 mL
	25 mM	0.0564 mL	0.2819 mL	0.5639 mL	1.4096 mL
	30 mM	0.0470 mL	0.2349 mL	0.4699 mL	1.1747 mL
	40 mM	0.0352 mL	0.1762 mL	0.3524 mL	0.8810 mL
	50 mM	0.0282 mL	0.1410 mL	0.2819 mL	0.7048 mL
	60 mM	0.0235 mL	0.1175 mL	0.2349 mL	0.5874 mL
	80 mM	0.0176 mL	0.0881 mL	0.1762 mL	0.4405 mL
	100 mM	0.0141 mL	0.0705 mL	0.1410 mL	0.3524 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

NADH disodium salt Related Classifications

Metabolic Disease

Metabolic Enzyme/Protease
Endogenous Metabolite

NADH (disodium salt) [606-68-8]

Cat# HY-F0001-500mg

Brand : MedChemExpress

Other Forms of NADH disodium salt:

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

NADH disodium salt Related Classifications

Keywords:

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NADH (disodium salt) [606-68-8]

Cat# HY-F0001-500mg

Brand : MedChemExpress

Other Forms of NADH disodium salt:

NADH disodium salt Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

NADH disodium salt Related Classifications

Keywords:

You might also be interested by the following products: