Mouse Anti-Plakoglobin Monoclonal Antibody (Clone:PG 5.1)
Cat# 690005
Size : 1ml(50µg/ml)
Brand : Progen
| Quantity | 1 ml |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG2b |
| Conjugate | Unconjugated |
| Application | ICC/IF, IHC, WB |
| Purification | Affinity chromatography |
| Reactivity | Bovine, Dog, Human, Mouse, Rat, Zebrafish |
| Storage | Short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/thaw cycles |
| Intended use | Research use only |
| Clone | PG 5.1 |
| Immunogen | Plakoglobin, "band 5" protein from bovine snout epidermis |
| Concentration | 50 µg/ml (50 µg) |
| Formulation | PBS pH 7.4 with 0.09% sodium azide and 0.5% BSA |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:5 (10 µg/ml) |
| Immunohistochemistry (IHC) - frozen | 1:25-1:100 (0.5-2 µg/ml) |
| Immunohistochemistry (IHC) - paraffin | 1:25-1:100 (0.5-2 µg/ml, microwave treatment recommended) |
| Western Blot (WB) | 1:500-1:2,000 (0.025-0.1 µg/ml) |
PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: desmosomes of epithelial and myocardial cells (incl. cultured cells); zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin. Polypeptide reacting: Mr 83,000 Plakoglobin, 'band 5' polypeptide of intercellular adhering junctions (identical to gamma-catenin).
Reactivity on cultured cell lines: cell cultures forming monolayers.
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