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> Isotype Control, Mouse IgG2b Kappa (IgG2b/1333), Purified, with BSA, 0.2 mg/mL

Isotype Control, Mouse IgG2b Kappa (IgG2b/1333), Purified, with BSA, 0.2 mg/mL

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Cat# BNUB1333-250

Size : 250uL

Brand : Biotium

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Isotype Control, Monoclonal Mouse IgG2b Kappa (IGG/1333)

Product Attributes

Antibody number

#1333
Antibody reactivity (target)

Isotype Control
Antibody type

Isotype Control
Host species

Mouse
Clonality

Monoclonal
Clone

IGG/1333
Isotype

IgG2b, kappa
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody application notes

For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

Primary & Secondary Antibody Conjugates

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Primary antibody formats:

  • CF® Dye, APC, R-PE, PerCP, biotin, and alkaline phosphatase conjugates: 0.1 mg/mL in PBS, 0.1% BSA, 0.05% sodium azide
  • Horseradish peroxidase: 0.1 mg/mL in PBS, 0.05% BSA, no sodium azide
  • Purified, with BSA: 0.2 mg/mL in PBS, 0.05% BSA, 0.05% sodium azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no sodium azide
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

This is a non-reactive mouse monoclonal IgG2b kappa antibody labeled with one of our bright and photostable CF® Dyes and other labels. This antibody may serve as a control for a mouse monoclonal IgG2b antibody under identical experimental conditions. Biotium also carries mouse monoclonal isotype control antibodies for IgG1 Kappa (IGG1/1331) and IgG2a Kappa (MG2a-53).

  • Isotype control for antibody-based experiments
  • Available with 6 bright and photostable CF® Dyes and biotin
  • Suitable for western, flow cytometry, ELISA, immunofluorescence,  and immunohistology

CF® Dyes offer exceptional brightness and photostability; learn more about CF® Dyes.

  Catalog number key for antibody number 1333, Anti-Isotype Control (IGG/1333) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405 CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC CF®568 Features
BNC94 CF®594 593/614 561 Texas Red® CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5 CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5 CF®647 Features
BNC74 CF®740 742/767 633-685 775/50 CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.