IPKine™ HRP, Goat Anti-Mouse IgG HCS
Cat# A25112-100
Size : 100uL
Brand : Abbkine
Specification
Product name | IPKine™ HRP, Goat Anti-Mouse IgG HCS |
Immunogen | Mouse IgG Fc Fragment |
Host | Goat |
Reactivity | Mouse |
Applications | ELISA, IHC-P, WB |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:10000), IHC-P (1:50-1:500), ELISA (1:500-1:10000). |
Clonality | Polyclonal |
Preparation method | Affinity purified using solid phase Mouse IgG(H+L) Fc Fragment with finally > 95% purity based on SDS-PAGE |
Conjugate | HRP |
Product Properties
Formulation | Liquid solution |
Features & Benefits | 50kD or 25kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination). Abbkine's IPKine™ products solve these problems through less background noise, and enhanced accuracy). Their light chain/Fc fragment specific optimization will eliminate heavy/light chain interference, while serum proteins absorbed minimize cross-reactivity with immunoglobulins from other species which may be present on blots). |
Storage buffer | Liquid in PBS, pH 7.4, containing 1% BSA as stablizer and 50% Glycerol. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
Background | Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. |
Others | The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins |
Image & description
-
Lipid overload-induced RTN3 activation leads to cardiac dysfunction by promoting lipid droplet biogenesis(A25112-12.4)
Author:Guo D, Zhang M, Qi B, et al Publication name:Cell Death & Differentiation IF:12.4
-
TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells(A25112-9.043)
Author: Jiang C, Ma N, Cao H Publication name:Microbiology Spectrum IF:9.043
-
hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility(A25112-8.8)
Author:Wang, **aoli, et al Publication name:Cell Reports IF:8.8
-
SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia/reperfusion injury(A25112-8.37)
Author:Z Wang, R Sun, G Wang Publication name:Redox biology IF:8.37
-
Nedd4-2 haploinsufficiency in mice causes increased seizure susceptibility and impaired Kir4.1 ubiquitination(A25112-7.7)
Author:X Liu, H Zhang, B Zhang Publication name:Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease IF:7.7
-
Ribosomal L1 domain-containing protein 1 coordinates with HDM2 to negatively regulate p53 in human colorectal Cancer cells(A25112-7.068)
Author:Ding L, Zhang Z, Zhao C, Chen L, Chen Z, Zhang J, Liu Y, Nie Y, He Y, Liao K, Zhang X, Publication name:J Exp Clin Cancer Res IF:7.068
-
DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B(A25112-7.01)
Author:Xiaoyu Tian, Pengli Zheng, Chenqian Zhou, et al Publication name:Autophagy IF:7.01
-
TRIM21 restricts influenza A virus replication by ubiquitination-dependent degradation of M1(A25112-6.7)
Author:L Lin, X Wang, Z Chen, T Deng, Y Yan, W Dong Publication name:plos pathogens IF:6.7
-
Deletion of African swine fever virus (ASFV) H240R gene attenuates the virulence of ASFV by enhancing NLRP3-mediated inflammatory responses(A25112-5.4)
Author:Huang, Li, et al. Publication name:Journal of Virology IF:5.4
-
CTLA-4 Facilitates DNA Damage–Induced Apoptosis by Interacting With PP2A(A25112-5.201)
Author:Yan Q, Zhang B, Ling X, Zhu B, Mei S, Yang H, Zhang D, Huo J, Zhao Z, Publication name:Front Cell Dev Biol IF:5.201
-
DHX38 enhances proliferation, metastasis, and EMT progression in NSCLC through the G3BP1-mediated MAPK pathway(A25112-4.80)
Author:Mi K, Zeng L, Chen Y Publication name:Cellular Signalling IF:4.80
-
C-Jun/C7ORF41/NF-κB axis mediates hepatic inflammation and lipid accumulation in NAFLD(A25112-4.05)
Author:FJ Yan, X Wang, SE Wang Publication name:Biochemical Journal IF:4.05
-
Peste des petits ruminants virus nucleocapsid protein inhibits interferon-β production by interacting with IRF3 to block its activation(A25112-4.02)
Author:Zixiang Zhu, Pengfei Li, Fan Yang, et al Publication name:J Virol IF:4.02
-
Expression level of 12-amino acid triggering receptor on myeloid cells-like transcript 1 derived peptide alleviates lipopolysaccharide-induced acute lung injury in mice(A25112-2.91)
Author:Shi R, Zhang J, Peng Z, et al Publication name:Int J Mol Med IF:2.91
Reviews
There are no reviews yet.