Reagents and instruments for immunology, cell biology and molecular biology.
 
> Human COL2α1(Collagen Type Ⅱ Alpha 1) ELISA Kit

Human COL2α1(Collagen Type Ⅱ Alpha 1) ELISA Kit

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Cat# E-EL-H0777-96T

Size : 96T

Brand : Elabscience

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Product Summary
Sensitivity 0.38 ng/mL
Detection Range 0.63-40 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Human
Specificity This kit recognizes Human COL2α1 in samples. No significant cross-reactivity or interference between Human COL2α1 and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃/-20℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL2α1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL2α1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL2α1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL2α1. You can calculate the concentration of Human COL2α1 in the samples by comparing the OD of the samples to the standard curve.
Uniport ID P02458
Research Area Cancer , Signal Transduction , Stem Cells
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