HA tag (7H5) Monoclonal Antibody
Cat# bsm-50131M
Size : 100ul
Brand : Bioss
HA tag (7H5) Monoclonal Antibody
Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2 : Bosc23 cell lysate transfected with Myc\/His\/HePTP; Lane 3 : Bosc23 cell lysate transfected with GFP\/Ub+1 lysates, probed with HA tag (7H5) Monoclonal Antibody, unconjugated (bsm-50131M) at 1:10000 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:5000 for 60 minutes at Room Temperature.
Immunoprecipitation of Bosc23 cell lysates using HA tag (7H5) \r\nMonoclonal Antibody (bsm-50131M): Lane 1 : Bosc23 cell lysate transfected with HA\/HSP60; Lane 2: Precipitated from 200ug Bosc23 transfected with HA\/HSP60 using 5ug of antibody; Lane 3 : Precipitated from PBS using 5ug of antibody; WB analysis was performed using bsm-50131M at 0.1ug\/mL (1:10000) and incubated at 4\u2103 overnight, followed secondary antibody incubation for 60min at Room Temperature.
Applications
Reactivity
| Overview | |
| Catalog # | bsm-50131M |
| Product Name | HA tag (7H5) Monoclonal Antibody |
| Applications | WB, IF(ICC), IP |
| Reactivity | Human, All |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Mouse |
| Source | Recombinant Human HA-tag protein purified E.coli |
| Clonality | Monoclonal |
| Clone # | 7H5 |
| Isotype | IgG1 |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Background | Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage. The expression vectors are used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. Short pieces of welldefined peptides(Poly-His, Flag-epitope or cmyc epitope or HA-tag) or small proteins (bacterial GST, MBP) is often cloned along with the target gene. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. It has been successfully used in a variety of applications, including Western blotting, Immunoprecipitation, and immunofluorescence studies. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IP | 1-2ug |