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> Goat anti-SDCCAG8

Goat anti-SDCCAG8

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Cat# EB11471

Size : 100ug

Brand : Everest Biotech

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Code Name Applications Availability Size Tested species Grade
EB11471 Goat Anti-SDCCAG8 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human
EB11471 1
EB11471 (0.3µg/ml) staining of HEK293 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB11471 2
EB11471 (5µg/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Target Protein

Principal Names: antigen NY-CO-8, BBS16, CCCAP, centrosomal colon cancer autoantigen protein, hCCCAP, NPHP10, NY-CO-8, OTTHUMP00000037913, SDCCAG8, serologically defined colon cancer antigen 8, SLSN7
Official Symbol: SDCCAG8
Accession Number(s): NP_006633.1
Human GeneID(s): 10806
Non-Human GeneID(s): 76816 (mouse), 305002 (rat)

Immunogen

Peptide with sequence C-TINDQSQYIHHLEAE, from the internal region of the protein sequence according to NP_006633.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 82.7kDa according to NP_006633.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig