Goat anti-SDCCAG8
Cat# EB11471
Size : 100ug
Brand : Everest Biotech
Code | Name | Applications | Availability | Size | Tested species | Grade |
---|---|---|---|---|---|---|
EB11471 | Goat Anti-SDCCAG8 Antibody | Pep-ELISA, WB, IHC | ![]() | 100µg specific antibody in 200µl | Human |
Target Protein
Principal Names: antigen NY-CO-8, BBS16, CCCAP, centrosomal colon cancer autoantigen protein, hCCCAP, NPHP10, NY-CO-8, OTTHUMP00000037913, SDCCAG8, serologically defined colon cancer antigen 8, SLSN7Official Symbol: SDCCAG8
Accession Number(s): NP_006633.1
Human GeneID(s): 10806
Non-Human GeneID(s): 76816 (mouse), 305002 (rat)
Immunogen
Peptide with sequence C-TINDQSQYIHHLEAE, from the internal region of the protein sequence according to NP_006633.1.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:128000.Western blot: Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 82.7kDa according to NP_006633.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Pig
- Western blot Guidelines
- Everest Western blot labeling
- Western blot Trouble shooting guide
- IHC staining on paraffin sections
- Immunofluorescence Protocol
- Flow Cytometry Protocol
- MSDS - All Goat antibodies
- Tissue Lysate Preparation
- Cell Lysate Preparation
- Blocking with the immunizing peptide
- LsBio IHC Protocol