Galanthus nivalis (Snowdrop Bulb) (GNA)

Galanthus nivalis lectin is a small molecular weight tetramer consisting of subunits of about 13,000D. It has an isoelectric point below pH 4.6. The lectin contains little or no carbohydrate and, unlike most mannose-specific lectins, is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (a-1,3) mannose residues. Also in contrast to most mannose-binding lectins, GNL will not bind alpha linked glucose. Reports indicate that this lectin binds IgM but not IgG of the immunoglobulin classes of rat and mouse. The only protein from human serum reported to bind to this lectin is a2-macroglobulin.||Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended Dilutions: |Optimal dilutions to be determined by the researcher.||Inhibiting/Eluting Sugar: |100mM-200mM a-methyl mannoside||Storage and Stability:|Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.