FDG - Galactosidase Assay Kit
Cat# CA083
Size : 500assays
Brand : Canvax Biotech
FDG – Galactosidase Assay Kit
Optimized, Stable & Convenient tool to quickly measure the levels of active β-galactosidase
FDG β-Galactosidase Assay Kit is an efficient, easy and highly sensitive tool to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Lac Z is often used reporter gene in experiments transfection because the β-galactosidase is very resistant to proteolytic degradation and its activity is easily measured. β-galactosidase performs the hydrolysis of fluorescein di-β-D galactopyranoside (FDG) into a fluorescein that can be detected at excitation/emission = 490/525 nm. The concentration of β-galactosidase is proportional to fluorescence produced.measure levels of active β-galactosidase expressed in cells transfected with plasmids expressing Lac Z.
Detailed information:
- Fast and convenient.
- Easy-to-use method to quantify the enzyme expression in transfected cells.
- Sensitive: measure ß-galactosidase at femtogram level.
– 55 mL Reaction Buffer
– 25 mL Lysis Buffer
– 25 mL Stop solution
– 20 μL β-galactosidase enzyme (0.1 mg/mL)
– 500 μL β-Mercaptoethanol
– 125 μL FDG solution (10 mM)
Datasheet MSDS- Measurement of β-Galactosidase activity in the lysates of transfected cell.
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- Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
- Shipped in: Gel Pack.
- Storage: Upon receipt, store the kit at -20 ºC. β-galactosidase substrate must be protected from light.
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
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