EasyScript® First-Strand cDNA Synthesis SuperMix
Cat# AE301-03
Size : 100rxns×20ulsystem
Brand : TransGen Biotech
ManualEasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.
• cDNA up to 8 kb.
Applications
Multiple copy gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
| Component | AE301-02 | AE301-03 |
| EasyScript® RT/RI Enzyme Mix | 50 μl | 100 μl |
| 2×ES Reaction Mix | 500 μl | 1 ml |
| Random Primer(N9) (0.1 μg/μl) | 50 μl | 100 μl |
| Anchored Oligo(dT)18 Primer (0.5 μg/μl) | 50 μl | 100 μl |
| RNase-free Water | 500 μl | 1 ml |
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