EasyPure® Blood Genomic DNA Kit (with RNase A）
Cat# EE121-01
Size : 50rxns
Brand : TransGen Biotech
ManualEasyPure® Blood Genomic DNA Kit provides a simple and convenient way to isolate high quality genomic DNA from 5-250 μl of fresh or frozen blood. Whole blood is incubated with binding/lysis buffer to release DNA. DNA is bound to silica-based column.
• Simple and fast, red cell lysis buffer is no longer needed.
• Complete removal of contaminants and inhibitors.
• DNA yield up to 40 μg.
• Column based purification, no organic extraction or ethanol precipitation.
• Suitable for EDTA, sodium citrate and heparin-anticoagulated fresh or frozen blood in a volume of 5 to 250 μl.
Applications
PCR, restriction enzyme digestion and Southern blot
Storage
At room temperature (15-25°C) in a dry place for one year
Shipping
at room temperature.
| Component | EE121-01 (50 rxns) | EE121-02(200 rxns) | EE121-11 (50 rxns) | EE121-12(200 rxns) |
| Binding Buffer 3 (BB3) | 30 ml | 110 ml | 30 ml | 110 ml |
| Clean Buffer 3 (CB3) | 6 ml | 24 ml | 6 ml | 24 ml |
| Wash Buffer 3 (WB3) | 12 ml | 2×22 ml | 12 ml | 2×22 ml |
| Elution Buffer (EB) | 25 ml | 80 ml | 25 ml | 80 ml |
| RNase A (20 mg/ml) | 500 μl | 2×1 ml | 0 | 0 |
| Proteinase K (20 mg/ml) | 1 ml | 4×1 ml | 1 ml | 4×1 ml |
| Genomic Spin Columns with Collection Tubes | 50 each | 200 each | 50 each | 200 each |
Li X , Zhu Y , Liu P , et al. Assessment of genetic diversity in Chinese eared pheasant using fluorescent-AFLP markers.[J]. Molecular Phylogenetics & Evolution, 2010, 57(1):429-433.
Zhang S , Wang Q M , Ding X P , et al. Association of polymorphisms in PATE1 gene with idiopathic asthenozoospermia in Sichuan, China[J]. Journal of Reproductive Immunology, 2016, 118(2):54-60.
Ablimit A , Qin W , Shan W , et al. Genetic diversities of cytochrome B in Xinjiang Uyghur unveiled its origin and migration history[J]. BMC Genetics, 2013, 14(1):100.
Xin C , Hao Y , Tong-Wen O , et al. Genetic Variations in Inflammatory Response Genes and Their Association with the Risk of Prostate Cancer[J]. BioMed Research International, 2015, 2015:1-7.
Lu Z H , Gu X J , Shi K Z , et al. Association between genetic polymorphisms of inflammatory response genes and the risk of ovarian cancer[J]. Journal of the Formosan Medical Association, 2016, 115(1):31-7.