Reagents and instruments for immunology, cell biology and molecular biology.

Dipeptidyl Peptidase IV (DPP4) Activity Fluorometric Assay Kit

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Cat# E-BC-F053-96T

Size : 96T

Brand : Elabscience

Detection Principle	Dipeptidyl Peptidase IV (DPP4), also known as CD26, is a kind of serine protease, can decompose the second N-terminal proline or alanine residue of the peptide chain. In organisms, dipeptidyl peptidase can rapidly decompose incretin, which can stabilize insulin level and promote the reduction of blood glucose level in organisms. The detection principle of this kit is that DPP4 can decompose the substrate and release the fluorescent substance AMC, and the activity of this enzyme can be inhibited by adding DPP4 inhibitor. The activity of DPP4 could be calculated by the difference of fluorescence value before and after inhibition.
Synonyms	DPP4
Sample Type	Serum,Plasma,Tissue,Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=360 nm/460 nm)
Research Area	Amino Acids And Proteins
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.92 U/L
Detection Range	0.92-3.77 U/L
Precision	inter-assay CV: 5.7% \| intra-assay CV: 0.6%
Assay Time	1 h 10 min

Cat.No.	Product Name	Sizes
E-BC-K234-M	Glucose (Glu) Colorimetric Assay Kit (GOD-POD Method)	500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
5% Mouse kidney tissue homogenate	4-5
5% Mouse lung tissue homogenate	4-5
5% Mouse heart tissue homogenate	4-5
5% Rat liver tissue homogenate	4-5
5% Rat brain tissue homogenate	4-5
Porcine serum	4-5
Horse serum	4-5
Dog serum	4-5
Female chicken serum	4-5
Macaca fascicularis serum	4-5
5.0 × 10^6 HL-60 cell	1
4.1 × 10^6 293T cell	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.