Reagents and instruments for immunology, cell biology and molecular biology.
 
> Cell Counting Kit-8 (CCK-8)

Cell Counting Kit-8 (CCK-8)

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Cat# KTA1020-1000T

Size : 1000T

Brand : Abbkine

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Specification

Product name Cell Counting Kit-8 (CCK-8)
Applications notes Cell Counting Kit-8 (CCK-8) is designed to detect cell proliferation and cell toxicity based on WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan). The amount of the formazan in cells is directly proportional to the number of living cells.
Alternative CCK-8; WST-8; CCK8; WST8

Product Properties

Formulation Liquid solution
Kit components solution
Features & Benefits is water soluble, No organic solvents or isotopes required. And the formazan is stable and safe.
is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
.
Usage notes assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals that affect dehydrogenase activity in viable cells may cause discrepancy between the actual viable cell number and the cell number determined using the CCK-8 assay.
hours) or a large number of cells (~105 cells/well) may be necessary.
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Storage instructions CCK-8 is stable for one year at 0-5-5 class="pro-form-title">Additional Information
Background Cell Counting Kit-8 (CCK-8) allows very convenient assays by utilizing highly water-soluble tetrazolium salt-WST-8.[2-(2- methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] produces a water-soluble formazan dye upon reduction in the presence of an electron mediator. CCK-8, being nonradioactive, allows sensitive colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays. WST-8 is reduced by dehydrogenases in cells to give an orange colored product (formazan), which is soluble in the tissue culture medium. The amount of the formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells. The product (formazan) produced by WST-8 is water soluble, no organic solvents or isotopes required. And the formazan is stable and safe. The detection sensitivity using CCK-8 is higher than assays using other tetrazolium salts such as MTT, XTT, MTS or WST-1.
Alternative CCK-8; WST-8; CCK8; WST8

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