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Calcium phosphate transfection is a common method for introducing DNA plasmid into eukaryotic cells. The principle is to mix phosphate buffer with CaCl2 solution containing DNA plasmid to form DNA-calcium phosphate precipitate. This precipitate can adhere to the cell and eventually transfer DNA into the target cell by endocytosis.
This method has been successfully used in many cell lines to obtain transient or stable transfection. For example, HEK293 cell is one of the most suitable cells for calcium phosphate transfection. Other common cells (such as Hela and CHO cell) are also suitable for calcium phosphate transfection, but the transfection efficiency is slightly lower than that of HEK293 cell. This kit is modified on the basis of the traditional calcium phosphate method to achieve higher transfection efficiency and lower cytotoxicity, which is suitable for most transfection needs of adherent cell or suspended cell.
Store the components at 4°C.
