ARHGEF7 (Rho Guanine Nucleotide Exchange Factor 7 , Beta-PIX, COOL1, DKFZp686C12170, DKFZp761K1021, KIAA0142, KIAA0412, Nbla10314, P50, P50BP, P85, P85COOL1, P85SPR, PAK3, PIXB)

Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions. The family consists of two isoforms, cool2/alpha pix and cool1/betaPix, the latter having two splice variants that vary in their carboxy termini (1). Cool1/betaPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization. Cool1/betaPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4). Phosphorylation of cool1/betaPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/betaPix, regulating formation of growth cones in response to growth factors (4). Growth factor induced activation of Rac1 via cool1/betaPix was later shown to occur independently of subcellular localization (5). Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/betaPix and activation of cdc42 (6).||Applications: |Suitable for use in Western Blot. Other applications not tested.||Recommended Dilution:|Western Blot: 1:500-1:2000|Optimal dilutions to be determined by the researcher.||Storage and Stability: |May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.