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> Anti-SARS-CoV-2 Spike RBD antibody (332-550)

Anti-SARS-CoV-2 Spike RBD antibody (332-550)

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Cat# NB-22-48211-100

Size : 100uL

Brand : Neo Biotech

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  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Spike RBD antibody . Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike RBD antibody at a dilution of 1:3000.
  • Immunofluorescence analysis of 293T-RBD and 293T cells using SARS-CoV-2 Spike RBD Rabbit polyclonal antibody at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immobilized Recombinant SARS-COV-2 Spike RBD Protein (RP01278LQ) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike RBD Rabbit polyclonal antibody with a linear range of 0. 78-50ng/mL.
  • Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike RBD Protein, using SARS-CoV-2 Spike RBD Rabbit polyclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

General Info

Host: Rabbit
Applications: WB/IF/IP
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2 Spike RBD (332-550) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:50000-1:200000
WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:1000-1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Immunogen Region: 332-550
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 332-550 of coronavirus Spike RBD (YP_009724390.1).
Immunogen Sequence: ITNLCPFGEVFNATRFASVY AWNRKRISNCVADYSVLYNS ASFSTFKCYGVSPTKLNDLC FTNVYADSFVIRGDEVRQIA PGQTGKIADYNYKLPDDFTG CVIAWNSNNLDSKVGGNYNY LYRLFRKSNLKPFERDISTE IYQAGSTPCNGVEGFNCYFP LQSYGFQPTNGVGYQPYRVV VLSFELLHAPATVCGPKKST NLVKNKCVNFNFNGLTGTG

Description

Background This antimicrobial gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signals through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression of this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance