Anti-Phospho-MED1-Thr1457 antibody (1423-1472 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MED1
Gene ID:	5469
Uniprot ID:	MED1_HUMAN
Immunogen Region:	1423-1472 aa
Specificity:	Phospho-TRAP220 (T1457) Polyclonal Antibody detects endogenous levels of TRAP220 protein only when phosphorylated at T1457.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PPAR-BP around the phosphorylation site of Thr1457 at the amino acid range 1423-1472

Post Translational Modifications	Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1.
Function	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.
Protein Name	Mediator Of Rna Polymerase Ii Transcription Subunit 1 Activator-Recruited Cofactor 205 Kda Component Arc205 Mediator Complex Subunit 1 Peroxisome Proliferator-Activated Receptor-Binding Protein Pbp Ppar-Binding Protein Thyroid Hormone Receptor-Associated Protein Complex 220 Kda Component Trap220 Thyroid Receptor-Interacting Protein 2 Tr-Interacting Protein 2 Trip-2 Vitamin D Receptor-Interacting Protein Complex Component Drip205 P53 Regulatory Protein Rb18a
Database Links	Reactome: R-HSA-1368082 Reactome: R-HSA-1368108 Reactome: R-HSA-1989781 Reactome: R-HSA-212436 Reactome: R-HSA-2151201 Reactome: R-HSA-2426168 Reactome: R-HSA-381340 Reactome: R-HSA-383280 Reactome: R-HSA-400206 Reactome: R-HSA-400253 Reactome: R-HSA-9018519 Reactome: R-HSA-9707564 Reactome: R-HSA-9707616
Cellular Localisation	Nucleus A Subset Of The Protein May Enter The Nucleolus Subsequent To Phosphorylation By Mapk1 Or Mapk3
Alternative Antibody Names	Anti-Mediator Of Rna Polymerase Ii Transcription Subunit 1 antibody Anti-Activator-Recruited Cofactor 205 Kda Component antibody Anti-Arc205 antibody Anti-Mediator Complex Subunit 1 antibody Anti-Peroxisome Proliferator-Activated Receptor-Binding Protein antibody Anti-Pbp antibody Anti-Ppar-Binding Protein antibody Anti-Thyroid Hormone Receptor-Associated Protein Complex 220 Kda Component antibody Anti-Trap220 antibody Anti-Thyroid Receptor-Interacting Protein 2 antibody Anti-Tr-Interacting Protein 2 antibody Anti-Trip-2 antibody Anti-Vitamin D Receptor-Interacting Protein Complex Component Drip205 antibody Anti-P53 Regulatory Protein Rb18a antibody Anti-MED1 antibody Anti-ARC205 antibody Anti-CRSP1 antibody Anti-CRSP200 antibody Anti-DRIP205 antibody Anti-DRIP230 antibody Anti-PBP antibody Anti-PPARBP antibody Anti-PPARGBP antibody Anti-RB18A antibody Anti-TRAP220 antibody Anti-TRIP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance