Alpha-1-microglobulin BioAssay™ ELISA Kit (Hamster)

This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.||Sample Type:|Serum, plasma and tissue homogenates||Intended Use:|For the quantitative determination of hamster alpha-1-microglobulin/bikunin precursor (AMBP) concentrations in serum, plasma, tissue homogenates.||Sensitivity:|0.649ng/ml||Range:|1.563-100ng/ml||Specificity:|This assay has high sensitivity and excellent specificity for detection of hamster AMBP. No significant cross-reactivity or interference between hamster AMBP and analogues was observed.||Test Principle:|This BioAssay™ employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AMBP. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for AMBP. The competitive inhibition reaction is launched between with pre-coated AMBP and AMBP in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of AMBP in the samples. The color development is stopped and the intensity of the color is measured.||Kit Components:|Assay plate, 1x96 wells|Standard (Freeze dried), 2x vials|HRP-conjugate (100X), 1x60ul|HRP-conjugate Diluent, 1x10ml|Sample Diluent, 2x20ml|Wash Buffer (25X), 1x20ml|TMB Substrate, 1x10ml|Stop Solution, 1x10ml|Adhesive Strip (For 96 wells), 4x strips|Instruction manual||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.