General Info
| Applications: | ELISA |
| Reactivity: | Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 1.38ng/mL |
| Detection Limit: | 3.12-200ng/mL |
| Short Description: | This FADS2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
Information
| Gene Symbol: | Fads2 |
| Gene ID: | 83512 |
| Uniprot ID: | FADS2_RAT |
| Sample Type: | serum, plasma, tissue homogenates or other biological fluids. |
Description
| Tissue Specificity | Expressed in the liver and brain (at protein level). Highest activity is found in the liver and adrenals followed by the testes and other organs, absent in adipose tissue. |
| Post Translational Modifications | |
| Function | Involved in the biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors, acting as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively. Subsequently, in the biosynthetic pathway of HUFA n-3 series, it desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function. It can also desaturate (11E)-octadecenoate (trans-vaccenoate) at carbon 6 generating (6Z,11E)-octadecadienoate. In addition to Delta-6 activity, this enzyme exhibits Delta-8 activity with slight biases toward n-3 fatty acyl-CoA substrates. |
| Protein Name | Acyl-Coa 6-DesaturaseDelta(6 Fatty Acid DesaturaseD6dDelta(6 DesaturaseDelta-6 DesaturaseFatty Acid Desaturase 2 |
| Database Links | Reactome: R-RNO-2046105Reactome: -RNO-2046106 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane ProteinMicrosome Membrane |
| Alternative ELISA Names | Acyl-Coa 6-Desaturase ELISA kitDelta(6 Fatty Acid Desaturase ELISA kitD6d ELISA kitDelta(6 Desaturase ELISA kitDelta-6 Desaturase ELISA kitFatty Acid Desaturase 2 ELISA kitFads2 ELISA kitFadsd6 ELISA kit |
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Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance