Reagents and instruments for immunology, cell biology and molecular biology.

Anti-STAT1 antibody (694-743 aa)

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Cat# NB-22-5802-50

Size : 50ug

Brand : Neo Biotech

Western blot analysis of lysates from 293/3T3/Jurkat, using STAT1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of various cells using Stat1 Polyclonal Antibody diluted at 1：2000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using STAT1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Stat1 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of human-stomach tissue. 1, Stat1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-liver tissue. 1, Stat1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Hela cell. 1, Stat1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Rabbit
Applications:	IF/WB/IHC/IP/ELISA
Reactivity:	Human/Mouse/Rat/Chicken
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Signal transducer and activator of transcription 1-alpha/beta (694-743 aa) is suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IF 1:50-200 WB 1:500-1:2000 IHC 1:100-1:300 IP 1:200 ELISA 1:10000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	STAT1
Gene ID:	6772
Uniprot ID:	STAT1_HUMAN
Immunogen Region:	694-743 aa
Specificity:	Stat1 Polyclonal Antibody detects endogenous levels of Stat1 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human STAT1 at the amino acid range 694-743

Description

Function	Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Involved in food tolerance in small intestine: associates with the Gasdermin-D, p13 cleavage product (13 kDa GSDMD) and promotes transcription of CIITA, inducing type 1 regulatory T (Tr1) cells in upper small intestine.
Protein Name	Signal Transducer And Activator Of Transcription 1-Alpha/Beta Transcription Factor Isgf-3 Components P91/P84
Database Links	Reactome: R-HSA-1059683 P42224-1 Reactome: R-HSA-1169408 Reactome: R-HSA-1433557 P42224-1 Reactome: R-HSA-1839117 Reactome: R-HSA-186763 Reactome: R-HSA-2173795 P42224-1 Reactome: R-HSA-6785807 Reactome: R-HSA-877300 P42224-1 Reactome: R-HSA-877312 P42224-1 Reactome: R-HSA-8854691 Reactome: R-HSA-8939902 Reactome: R-HSA-8984722 Reactome: R-HSA-8985947 Reactome: R-HSA-9013508 Reactome: R-HSA-9020956 Reactome: R-HSA-9020958 Reactome: R-HSA-909733 Reactome: R-HSA-912694 Reactome: R-HSA-9670439 P42224-1 Reactome: R-HSA-9673767 Reactome: R-HSA-9673770 Reactome: R-HSA-9674555 Reactome: R-HSA-9680350 Reactome: R-HSA-9705462 Reactome: R-HSA-9705671 Reactome: R-HSA-982772
Cellular Localisation	Cytoplasm Nucleus Translocated Into The Nucleus Upon Tyrosine Phosphorylation And Dimerization In Response To Ifn-Gamma And Signaling By Activated Fgfr1 Fgfr2 Fgfr3 Or Fgfr4 Monomethylation At Lys-525 Is Required For Phosphorylation At Tyr-701 And Translocation Into The Nucleus Translocates Into The Nucleus In Response To Interferon-Beta Stimulation
Alternative Antibody Names	Anti-Signal Transducer And Activator Of Transcription 1-Alpha/Beta antibody Anti-Transcription Factor Isgf-3 Components P91/P84 antibody Anti-STAT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance