Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Histone H3 antibody (1-50 aa)

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Cat# NB-22-3526-50

Size : 50ug

Brand : Neo Biotech

Western blot analysis of lysates from COLO205 cells, using Histone H3.1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human testis. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Western blot analysis of customer's (cat sample) using Histone H3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Western blot analysis of MCF-7 cells using Histone H3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Western blot analysis of various cells using Histone H3 Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
Immunofluorescence analysis of Hela cell. 1, Histone H3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). LC3A mouse monoclonal antibody (5G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat/Cat/Rice
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Histone H3.1 and Histone H3.2 and Histone H3.3 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:20000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	H3C15.H3C14.H3C13 H3-3A.H3-3B 126961/333932/653604 3020/3021 H32_HUMAN H33_HUMAN H31_HUMAN <
Immunogen Region:	1-50 aa
Specificity:	Histone H3 Polyclonal Antibody detects endogenous levels of Histone H3 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human Histone H3.1 at the amino acid range 1-50

Description

Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name	Histone H3.1 Histone H3/A Histone H3/B Histone H3/C Histone H3/D Histone H3/F Histone H3/H Histone H3/I Histone H3/J Histone H3/K Histone H3/L
Database Links	Reactome: R-HSA-1266695 Reactome: R-HSA-1912408 Reactome: R-HSA-201722 Reactome: R-HSA-212300 Reactome: R-HSA-2299718 Reactome: R-HSA-2559580 Reactome: R-HSA-2559582 Reactome: R-HSA-3214815 Reactome: R-HSA-3214841 Reactome: R-HSA-3214842 Reactome: R-HSA-3214847 Reactome: R-HSA-3214858 Reactome: R-HSA-3247509 Reactome: R-HSA-427359 Reactome: R-HSA-427389 Reactome: R-HSA-427413 Reactome: R-HSA-5250924 Reactome: R-HSA-5334118 Reactome: R-HSA-5578749 Reactome: R-HSA-5617472 Reactome: R-HSA-5625886 Reactome: R-HSA-73728 Reactome: R-HSA-73772 Reactome: R-HSA-8936459 Reactome: R-HSA-8939236 Reactome: R-HSA-9018519 Reactome: R-HSA-912446 Reactome: R-HSA-9609690 Reactome: R-HSA-9610379 Reactome: R-HSA-9616222 Reactome: R-HSA-977225 Reactome: R-HSA-983231
Cellular Localisation	Nucleus Chromosome
Alternative Antibody Names	Anti-Histone H3.1 antibody Anti-Histone H3/A antibody Anti-Histone H3/B antibody Anti-Histone H3/C antibody Anti-Histone H3/D antibody Anti-Histone H3/F antibody Anti-Histone H3/H antibody Anti-Histone H3/I antibody Anti-Histone H3/J antibody Anti-Histone H3/K antibody Anti-Histone H3/L antibody Anti-H3C1 antibody Anti-H3FA antibody Anti-HIST1H3A.H3C2 antibody Anti-H3FL antibody Anti-HIST1H3B.H3C3 antibody Anti-H3FC antibody Anti-HIST1H3C.H3C4 antibody Anti-H3FB antibody Anti-HIST1H3D.H3C6 antibody Anti-H3FD antibody Anti-HIST1H3E.H3C7 antibody Anti-H3FI antibody Anti-HIST1H3F.H3C8 antibody Anti-H3FH antibody Anti-HIST1H3G.H3C10 antibody Anti-H3FK antibody Anti-HIST1H3H.H3C11 antibody Anti-H3FF antibody Anti-HIST1H3I.H3C12 antibody Anti-H3FJ antibody Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance