Mouse Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) ELISA Kit
Cat# KTE70285-48
Size : 48T
Brand : Abbkine
Specification
| Product name | Mouse Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) ELISA Kit |
| Reactivity | Mouse |
| Applications | ELISA |
| Applications notes | This Mouse Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNCRIP in samples. An antibody specific for SYNCRIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNCRIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNCRIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNCRIP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Other biological fluids, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person. |
| Alternative | SYNCRIP; FLJ31626; GRY-RBP; GRYRBP; HNRPQ1; NSAP1; PP68; dJ3J17.2; hnRNP-Q; NS1-associated protein 1; glycine- and tyrosine-rich RNA-binding protein; heterogeneous nuclear ribonucleoprotein Q |
Product Properties
| Kit components | • Mouse Heterogeneous nuclear ribonucleoprotein Q microplate • Mouse Heterogeneous nuclear ribonucleoprotein Q standard • Mouse Heterogeneous nuclear ribonucleoprotein Q detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers |
| Features & Benefits | Mouse Heterogeneous nuclear ribonucleoprotein Q (SYNCRIP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNCRIP. No significant cross-reactivity or interference between Mouse SYNCRIP and analogues was observed. |
| Calibration range | Please inquire |
| Limit of detection | Please inquire |
| Usage notes | • Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate. |
| Storage instructions | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | SYNCRIP detected in heart, brain, pancreas, placenta, spleen, lung, liver, skeletal muscle, kidney, thymus, prostate, uterus, small intestine, colon, peripheral blood and testis.Heterogenous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Isoform 1, isoform 2 and isoform 3 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences. Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself. May be involved in translationally coupled mRNA turnover. |
| Gene ID | 56403 |
| Alternative | SYNCRIP; FLJ31626; GRY-RBP; GRYRBP; HNRPQ1; NSAP1; PP68; dJ3J17.2; hnRNP-Q; NS1-associated protein 1; glycine- and tyrosine-rich RNA-binding protein; heterogeneous nuclear ribonucleoprotein Q |
| Accession | Q7TMK9 |
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